Confocal microscopy

Zeiss LSM 880 

(inverted configuration)

Features include: 

• Fluorescence confocal, Brightfield imaging and data capture

• Inverted AxioObserver microscope stand

• Computer controlled by ZEN (Zeiss Enhanced Navigation) software

• Laser excitations at 405, 458, 488, 514, 561, 594 and 633 nm

• Available lenses: 10x, 20x, 25x, 40x and 63x (All DIC)

• X-Y Motorized stage for tiling and multiple region imaging

• High sensitivity GAsP detector

• Dedicated “blue” and “red” PMTs for the low and high end of the spectrum

• Highly efficient light path

• Simultaneous Fluorescence and DIC imaging

• Experimental Designer for multi-dimensional acquisition

• Tiling Macro for large image regions

Zeiss LSM 880 with AiryScan, FAST Airyscan and 32-channel GaAsP detector array for spectral imaging

(inverted configuration)

Features include: 

• Fluorescence confocal, Brightfield imaging and data capture

• Inverted AxioObserver microscope stand

• Computer controlled by ZEN (Zeiss Enhanced Navigation) software

• Laser excitations at 405, 458, 488, 514, 561, 594 and 633 nm

• Available lenses: 10x, 20x, 25x, 40x, 63x  (All DIC)

• X-Y Motorized stage for tiling and multiple region imaging

• Incubation chamber and heating inserts with CO2 for live cell imaging

• High sensitivity 32 channel GAsP detector for visible spectrum detection and linear unmixing

• Dedicated “blue” and cooled “red” PMTs for the low and high end of the spectrum

• Highly efficient light path

• Simultaneous Fluorescence and DIC imaging in confocal mode

• Experimental Designer for multi-dimensional acquisition

• Tiling Macro for large image regions

• AIRYSCAN and FAST AIRYSCAN high resolution GaAsP detectors

Leica Stellaris 8 with FALCON (FAst Lifetime CONtrast) and tunable White Light Laser illumination

(inverted configuration)

Features include: 

• White Light Laser (440nm-795nm emissions)

• FALCON TauSense technology for Fast Lifetime Imaging

• 5 High sensitivity HyD confocal fluorescence detectors

• FRET, FRAP: quantify fast cellular processes, like molecular interaction or molecular environment changes

• Solid state 405 nm laser

• Transmitted light detector

• Resonant and non-resonant scanning modes for optimal scanning speeds

• Motorized stage for X-Y tiling and Z-stack collection

• Automatic Focus Control (AFC)

• Fluorescence confocal, Brighfield imaging and data collection

• Inverted DMi 8 Premium stand

• LASX Software

Laser excitations: White Light laser (tunable from 440 - 790 nm); solid state 405 nm laser

Objectives: CS2 /low background lenses: 5x, 10x, 20x,40x, 63x-Oil-DIC, 63x-Glyc-DIC (four dry lenses 5 - 40x allow multi-position time-lapse imaging with more ease)

Leica SP8 with DIVE optics (combined confocal and multiphoton)

(upright configuration)

Features include:

• 3 confocal (descanned) detectors - two HyD and one PMT

• "Filterless" acquisition (custom emission bandwidths)

• “Navigator” for mark and find, and tile scanning

• Switchable between resonant and non-resonant mode for optimal scanning speeds and resolution

• Adjustable height fixed stage, along with a smaller motorized XY stage

Laser excitations: 405, 458, 488, 522, 638 nm. 

Objectives: 5x Air, 10x Air, 20x Air, 40x Oil, 63x Oil Immersion. All DIC for simultaneous transmitted light imaging.

For the multiphoton options on this instrument, please click the button below.

ImageXpress Micro Confocal Imager (automated spinning disk confocal)

(inverted configuration)

Features include: 

• Automated imaging of fixed or live cells in multi-well plates or glass slides

• Widefield imaging or spinning disk confocal imaging with 42µm and 60µm pinholes

• Solid-state light engine and filter sets for DAPI, FITC, TRITC, TxRed and Cy5 fluorescence

• High quantum efficiency 16-bit sCMOS camera enabling >3 log dynamic range and a large field of view (1.96 mm2 at 10X)

• Long working-distance objectives ranging from 2X to 40X

• Transmitted light and Phase Contrast imaging for non-fluorescent samples

• Multi-day, time-lapse imaging with temperature, humidity and CO2 control for live cells

• Unattended screening of up to 45 fixed plates per run

• Automated, fully customizable 2D and 3D image analysis on our MetaXpress workstations. Images can be exported for analysis in other packages

• Sample prep facilitated by two MultiDrop384 dispensers and a BioTek plate washer