Multiphoton Microscopy

Olympus FluoView FV1000MPE with Mai Tai DeepSee laser


Features include: 

  • fluorescence, autofluorescence and second harmonic imaging of cells, 3D cultures and thick tissues; 
  • intravital imaging (mice/rats); inhalation anesthesia equipment; 
  • oxygen supply; 
  • heated stage and circulating-water heating blankets; 
  • excitation laser tunable between 690 - 1040 nm; 
  • simultaneous detection of up to 4 signals; 
  • additional 405 nm laser (for uncaging and photoactivation). 


Objectives include: 

  • standard objectives (10x, 20x, 60x).
  • low-power large field-of-view macro lenses: 2x and 4x. 
  • IR-transmitting, high NA water-immersion objectives: 10x/0.6NA and 25x/1.05NA. 
  • Small tip diameter microprobe objectives: 6x and 20x; tip diameter: 1.3 mm; water-immersion.