Multiphoton Microscopy

Leica SP8 with DIVE optics

Leica SP8 with DIVE optics

Leica SP8 with DIVE optics (combined confocal and multiphoton)

(upright configuration)

Features include: 

  • fluorescence, autofluorescence and second harmonic imaging of cells, 3D cultures and thick tissues;

  • intravital imaging (mice/rats); inhalation anesthesia equipment;

  • oxygen supply;

  • heated stage and circulating-water heating blankets;

  • multiphoton excitation laser tunable between 690 - 1040 nm;

  • Confocal lasers include 405, 488, 514, 552 and 638 nm

  • simultaneous detection of up to 3 signals using “filterless” detector system (DIVE);

  • simultaneous confocal and multiphoton imaging possible

  • Single beam Multiphoton routing with Variable Beam Expander (to adjust filling of the objective back aperture for depth or resolution)

  • “Navigator” for mark and find, and tile scanning

  • Switchable between resonant and non-resonant mode for optimal scanning speeds and resolution

  • Adjustable height fixed stage, along with a smaller motorized XY stage

Objectives include: 

  • standard confocal objectives (5x dry, 10x dry. 20x dry, 40 x oil, 63x oil).

  • IR-transmitting, high NA water-immersion objective: 25X MotCorr (1.0NA)